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KMID : 0377819880080091117
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Diagnosis and Treatment
1988 Volume.8 No. 9 p.1117 ~ p.1123
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NEUROENDOCRIME REGULATION OF THYROTROPIN RELEASE IN CULTURED HUMAN PITUITARY CELLS
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Abstract
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The regulation of TSH release in man was investigated using cell cultures derived from human pituitan obtained within 24th of accidental death. TRH stimulated TSH release in a dose-dependent manner. The Edo was 2.9 0.6( SEND nmol/L, similar to that reported for rat pituitary cell cultures. The release of TSH was calcium de-pendent, since the calcium channel antagonist verapamil inhivited TRH-stimulated TSH release, and the calcium ionophore A23187 stimulated TSH re-lease. 12-)-Tetradecanoyl-phorbol-13-acetate stimulated TSH secretion, while dibuytryl cAMP had no effect. Epinephrine and serotonin stimulated TSH release, and dopamine and somatostatin inhibited TRH-stimulated TSH release. These findings have directly demonstrated that the regulation of TSH secretion by hypothalamic neuropept des aAd biogenic amines in the human pituitary is similar to that in the rat. The development of a tissue culture system to study thyrotrophs from postmortem human pituitaries provides the means for detailed studies of the regulation of TSH secretion in man. (J Clin Endocrinol Metab 65 :1159, 1987)
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KEYWORD
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